In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.
After Japan's defeat of Russia in the 1904 territorial war, W. E. B. Du Bois declared, "The Color Line in civilization has been crossed in modern times as it was in the great past. The awakening of the yellow races is certain. That the awakening of the brown and black races will follow in time, no unprejudiced student of history can doubt." Du…
Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject
Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification
Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy
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