Home » Nonfiction » Mathias Munschauer » High-Resolution Profiling of Protein-RNA Interactions

February 17 , 2010

High-Resolution Profiling of Protein-RNA Interactions


The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.

Download mobi

Read also

The Dragon Empress

From 1861 to 1908 a woman, the Empress Dowager Tz'u-hsi, born the daughter of a minor mandarin, held the supreme power in China. Opportunistic, ruthless, malicious, she ruled over four hundred million people. Marina Warner's biography lays bare her complex personality: her extreme conventionalism; her hatred of "foreigners"; her passion for power…


Show more...

How to download book

Buy this book

You can buy this book now only for $84.09. This is the lowest price for this book.

Buy book

Download book free

If you want to download this book for free, please register, approve your account and get one book for free.


After that you may download book «High-Resolution Profiling of Protein-RNA Interactions»:

Download Papyre FB2:


Download ePub:


Download MobiPocket:


Download Microsoft Reader (LIT):